Open source free software for a leica tcs sp5 confocal. Flexible automatic deformation monitoring software solution. The leica tcs sp5 mp covers a wide range of imaging applications multiphoton and one photon by combining two technologies in one system. Leica tcs sp2 switch on the lumen 200 fluorescence illumination system switch on the pc red rocker switch main on the desk a.
We realized that drivers for needed adaptec scsi boards should work and we do not see any obvious problem to shift lcs onto supported os win7. The leica tcs sp5 is supplied with the latest version of the licensed las af software. Leica confocal software lcs complements the optics and electronics by. Administration has basically walked away from the leica, telling people not to use it as it will be phased out and to favor the olympusthe increased demand for the olympus is leading to that being screwed up update. Leica tcs sp2 is a laser scanning confocal microscope with electronically. The lcs software provided by leica microsystems contains a frap application wizard that guides the user ot the leica tcs sp2 through the steps of a frap experiment. Application letters leica tcs sp2 and tcs sp2 aobs confocal express published 30 mars 2009 confocal microscopy, leica sp2. This can contain many different series acquired during the one experiment. A message will appear asking whether you want to initialize the stage. Since the lcs software is based on the user administration of windows. Tcs stands for true confocal scanner and sp5 for the 5 spectrophotometer channels this system is equipped with. Inverted laser scanning confocal microscope system with solent temperature controlled surround for live cell work.
While the leica tcs sp2 was stateoftheart when purchased, the system no longer meets our needs with respect to multilabel imaging studies, photosensitivity, imaging quality, speed, and versatility. To learn about the leica confocal software, use the book as a. Under the appearing menu bar, you find a leica tcs manual rev. You will discover useful features that you breezed over the first time. Details the leica tcs sp2 has a system magnification of 3. Integrates anywhere the leica tcs spe is compact and fits in every laboratory.
Prepare the cells in an imaging chamber 16 48 hours prior to the frap experiment. Furthermore, leica will cease to provide service for the sp2 within the next year. To maintain information on the most current level, the description of software functions was. Leica tcs sp2 aobs laser scanning spectral confocal microscope the leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. Leica tcs sp2, lcs and win7 confocal microscopy list.
The recent innovation from leica microsystems is a new lens dedicated to the work with glycerolwater or similarly embedded specimen. Leica tcs sp ii comes with a context sensitive online help system that teaches you the different functions of the system. Leica dm ire2 inverted microscope with fully motorized objective nosepiece and fluorescence filter cube change, brightfield and differential interference contrastdic transmitted light and incident light fluorescence. Leica infinity geospatial office software suite is designed to manage, process, analyse and quality check field survey data from uavs and terrestrial surveying equipment. Instructions for leica sp2 confocal equipped with visible. Leica tcs sp2 microscopy centre rijksuniversiteit groningen.
With its proven, highefficiency sp detection five channels simultaneously. Beckman institute for advanced science and technology. The leica tcs spes streamlined approach makes it accessible to every user, beginners and experts alike. This will create individual directories that can be viewed only by the respective user. At present, remotecare is available for the leica asp200 s and asp300 s tissue processors, the cm3600 xp cryomacrotome and the leica tcs sp5 confocal laser scanning microscope. Software for performance of physiological measures. To start the tutorial, choose help help in the tcsnt control program. An acoustooptical tunable filter aotf controls the intensity of the visible excitation laser light.
Leica confocal tcs sp2 this is part of the backprojected pinhole calculator. Instead, you are referred to the online help of the leica application suite. Leica tcs sp2 confocal microscopes products home leica leica microsystems. The operating software offers frap and fret with built in wizards to help. Leica tcs sp2 aobs laser scanning spectral confocal microscope.
Select machine for the configuration and dmi8 for the microscope. The system provides the full range of scan speeds at the highest resolution. Its an online workbook, because you can practically enforce the steps that make up the tutorial to learn how to use the leica tcs software. We have used leicas tcs sp2 laser scanning spectral confocal microscope for more than a year. Turn on the ctr control box, el6000fluorescent light source for the.
Details the leica tcssp2 has a system magnification of 3. Leica tcs sp2 aobs laser scanning spectral confocal. View online or download leica tcs sp5 x user manual, manual. The leica tcs sp2 system was designed for confocal recording and quantitative measurement via laser point scanning of fluorescence labeled living and fixed specimens. Leica tcs sp2 aobs confocal scanner mounted on a leica dm ire2 inverted microscope 405, 458, 476, 488, 496, 514, 543, 594. Tools for connecting autodesk civil 3d or revit directly with your leica field solutions for free.
Improved fieldtofinish from captivate to carlson office. Leica microsystems has developed different types of objective lenses. This laser based system is ideal for fixed photostable applications where high confocal resolution is critical. Superior image performance, all the configurable as a personal. Software for exploration control, multidimensional series acquisition x, y, z, t. Tcs sp2 laser scanning spectral confocal microscope from leica. My question is if there is anybody who has any experience with running acquistion software lcs on either 32bit or 64bit windows 7.
Please contact your sales person with possible substitutions. Leica confocal software, use the book as a tutorial. Oil lenses for thin fixed specimens and water lenses for living specimens in aqueous solutions. The software interface minimizes training time and allows the system to be up and running immediately. The leica d flim an mp flim systems are addons of the tcs sp2 and tcs sp5 laser scanning microscopes. Leica tcs sp2 confocal microscope zmbh imaging facility short descripon. Switch on the confocal scanner red rocker switch b. Software license the software described in this document is furnished under a license agreement which is included with the product.
Let the uvlamp for optical control and the lasers warm. This handbook should be considered a supplement to the handbooks of the leica tcs sp2, leica tcs sp5, and the bh tcspc. Tcs software with 3d capacity dongle with the pc pc hp sw 6600 workstation. This will activate the pc, the monitors, the microscope controller and blue hene laser. The leica lcs software can perform multidimensional image processing, fret, frap, and lambda scanning as well as controlling the motorized leica dm.
Superior image performance, all the configurable as a personal, multiuser or advanced imaging center multiple innovations from the pacesetter in confocal microscopy prepared for the future. To maintain information on the most current level, the description of software functions was intentionally omitted from these operating instructions. True confocal scanning tcs is a technique, where only a single, diffraction limited spot is illuminated and observed at a time. Leica dm ire2 inverted microscope with fully motorized objective nosepiece and fluorescence filter cube change. We are going to upgrade our acquisition computer serving our leica tcs sp2 system with a new motherboard, intel i5 core, two new ssd disks and win 7 os. Dmire2 inverted microscope objectives hc pl apo cs 10x0,30 dry 11 mm. Drawingalineontheannotationsheet240 drawingarectangleontheannotationsheet240. The benefit of confocal imaging is a dramatically increased contrast by removal of outoffocus haze. Leica cs20 field controller honoured with red dot award. Leica tcs sp2 laser scanning spectral confocal microscope part. The online help system opens with an activated contents window. The leica tcs sp2 is a point scanning confocal microscope, based around an.
To join, leave or search the confocal microscopy listserv, go to. It is recommended setting up a separate user id for each user system administrator. A software module for easy and fast navigation over larger samples. Online tutorial leica tcs comes with an online tutorial that teaches you how to use the software and hardware. The leica tcs sp5 is supplied with the latest version of the licensed leica application suite advanced fluorescence. The tcs sp2 is the perfect instrument for biomedical research, chemical analysis and material science applications. This leica sp2 platform is equipped with two leica confocal microscopes. To retrieve the contents of the online help system. The leica tcs sp8 sted 3x leica microsystems, germany is a highly versatile and advanced high end system from leica microsystems equipped with three hyds, two pmts and one transmission detector. To always keep the information up to date, the description of software functions was intentionally omitted from these operating instructions.
Very soon the rapid tissue processor leica peloris and the confocal laser scanning microscope leica sp2 will be added to the range. Application letters leica tcs sp2 and tcs sp2 aobs confocal. Leica sp2 experiments are saved as multiple tiff files in a single folder. Leica dmi8 advanced inverted light microscope with motorized stage. Dec 11, 2006 we have used leicas tcs sp2 laser scanning spectral confocal microscope for more than a year. The leica tcs sp2 true confocal scanner system combines microscopy and video technology with computer electronics, optics and laser technology to offer unprecedented two and threedimensional imaging. Leica tcs sp8 confocal microscope system andrew lackner. The leica tcs sp2 capability of switching between two laser intensities enables even more sophisticated experiments. Leica confocal tcssp2 this is part of the backprojected pinhole calculator. This agreement specifies the permitted and prohibited uses of the product.
The spatial calibration should be imported correctly from your iplab software. Leica tcs sp2 confocal inverted mrc centre for regenerative. Confocal microscopes from leica microsystems are partners in top level biomedical research and surface analysis in material science applications, offering unprecedented precision in threedimensional imaging and exact examination of subcellular structures and dynamic processes. These 3 lasers allow triple labeling using most common fluorescent labels which emit green, red or far red light. Leica tcssp8 confocal lsm university of new brunswick.
Confocal microscopy list leica tcs sp2, lcs and win7. Microscopy and microanalysis facility university of new brunswick. This activates only the air cooling of the argon laser. The leica tcs sp5 confocal fully covers a broad range of requirements in confocal and multiphoton imaging with excellent overall performance. The leica lcs software can perform multidimensional image processing, fret, frap, and lambda scanning as well as controlling the motorized leica dm ire2 microscope. However, the side length of its square pinhole is usually specified in airy disk units, referenced to a standard fixed wavelength of 580 nm, making it independent of the actual overall magnification. Year 2003, new leica tcs sp2 aobs visuv confocal microscope. Scsi interface to pc, supported by directaccess control panel\. Application letters leica tcs sp2 and tcs sp2 aobs. Refurbished leica tcs sp2 true confocal scanner system. With the las x control software, leica microsystems offers an interface, which shows all you need to know about your confocal on one screen. Double click on the las x icon to start the software. The microscope is a leica dmi 6000 cs inverted microscope with a motorized xystage and zdrive motor focus on the microscope stand 100 nm step size as well as a superz galvo stage 1500. Fluorescence recovery after photobleaching with the leica.
Laser scanning spectral confocal microscope superior image performance more results in less time. Leica tcs sp2 rs, laser scanning spectral confocal microscope parts. Las af 3 intuitive software that makes your life easier 24 leica tcs sp8 x freely tunable excitation 26 leica tcs sp8 sted opening the gate to superresolution 28 leica tcs sp8 mp look deeper into tissues with multiphoton microscopy 30 leica tcs sp8 cars imaging native specimens without dyes 32. The system is intuitively controlled and even users, who are new to confocal microscopy, will get publicationquality results immediately.
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